Through games of Feb. 25, 2001:
| # | Team | W-L | Pts. | Wk 13 |
| 1 | Chicago (16) | 22-3 | 608 | 3 |
| 2 | Wooster (4) | 24-3 | 583 | 5 |
| 3 | Ohio Northern (4) | 24-2 | 529 | 6 |
| T4 | Carthage | 21-4 | 528 | 1 |
| T4 | Wittenberg | 23-3 | 528 | 2 |
| 6 | Calvin | 22-4 | 482 | 7 |
| 7 | Wilkes (1) | 23-2 | 476 | 8 |
| 8 | Washington U. | 22-3 | 469 | 4 |
| 9 | Elmhurst | 21-4 | 427 | 9 |
| 10 | Christopher Newport | 23-3 | 402 | 10 |
| 11 | William Paterson | 22-4 | 377 | 11 |
| 12 | Wartburg | 24-3 | 328 | 13 |
| 13 | Mass-Dartmouth | 24-2 | 322 | 12 |
| 14 | Catholic | 22-5 | 281 | 15 |
| 15 | McMurry | 23-3 | 262 | 16 |
| 16 | Hampden-Sydney | 23-4 | 211 | 22 |
| 17 | Illinois Wesleyan | 19-6 | 196 | 24 |
| 18 | Widener | 21-5 | 192 | 20 |
| 19 | St. John's | 21-5 | 169 | --- |
| 20 | Lewis & Clark | 20-6 | 139 | --- |
| 21 | Linfield | 20-5 | 100 | 14 |
| 22 | UW-Eau Claire | 18-9 | 89 | --- |
| 23 | Gustavus Adolphus | 21-5 | 83 | 18 |
| 24 | Maryville (Tenn.) | 22-4 | 81 | 25 |
| 25 | Mississippi College | 22-4 | 72 | 17 |
Dropped out: No. 19 Roanoke; No. 21 UW-Stevens Point; No. 23 UW-Whitewater
Others receiving votes: Clark 45; Roanoke 42; Salem State 31; Amherst 16; Augustana 15; Buena Vista 8; Grinnell 6; Benedictine 5; Savannah Art and Design 5; Cabrini 4; Colby-Sawyer 4; UW-Stevens Point 4; Chapman 2; Elizabethtown 2; Brockport State 1; Hobart 1.
The D3hoops.com Top 25 is voted on by a panel of 25 coaches, Sports Information Directors and media members from across the country, and is published weekly.