Entering the 2000-01 season:
| # | Team | W-L | Pts. |
| 1 | Calvin (13) | 30-2 | 579 |
| 2 | William Paterson (9) | 23-5 | 561 |
| 3 | Wooster | 26-3 | 447 |
| 4 | Carthage (2) | 22-4 | 395 |
| 5 | UW-Stevens Point | 25-5 | 394 |
| 6 | Buena Vista (1) | 27-2 | 353 |
| 7 | Catholic | 24-5 | 350 |
| 8 | Chicago | 23-4 | 343 |
| 9 | Cortland State | 24-5 | 329 |
| 10 | St. John's | 20-7 | 323 |
| 11 | McMurry | 27-2 | 309 |
| 12 | Roanoke | 24-5 | 263 |
| 13 | Salem State | 27-5 | 258 |
| 14 | Widener | 24-4 | 253 |
| 15 | UW-Eau Claire | 27-6 | 243 |
| 16 | Rowan | 21-4 | 234 |
| T17 | Franklin and Marshall | 25-7 | 219 |
| T17 | Connecticut College | 19-5 | 219 |
| 19 | Wilkes | 16-9 | 200 |
| 20 | Christopher Newport | 25-3 | 176 |
| 21 | Lewis and Clark | 20-5 | 126 |
| 22 | Maryville (Tenn.) | 25-4 | 112 |
| 23 | Nebraska Wesleyan | 17-9 | 111 |
| 24 | Springfield | 21-9 | 109 |
| 25 | Illinois Wesleyan | 17-8 | 104 |
Others receiving votes: Williams 91; Hope 86; Otterbein 85; St. Thomas 70; Hamilton 66; Trinity (Texas) 58; Hampden-Sydney 52; Washington U. 50; Ohio Northern 49; DePauw 48; Amherst 47; Claremont-Mudd-Scripps 39; Wittenberg 29; Manchester 28; Rochester Tech 28; Lake Forest 27; Linfield 27; Penn State-Behrend 27; Bridgewater (Va.) 25; Scranton 23; Tufts 21; Gustavus Adolphus 15; St. John Fisher 14; Mt. St. Joseph 11; UW-Superior 11; Johns Hopkins 8; UW-Platteville 8; Muskingum 8; Western New England 7; Richard Stockton 6; Franklin 6; North Park 6; Albright 6; College of New Jersey 6; North Carolina Wesleyan 5; Marymount 4; Benedictine 4; UW-Whitewater 4; Hanover 4; Wheaton (Ill.) 3; Clark 2; New Jersey City 1.
The D3hoops.com Top 25 is voted on by a panel of 25 coaches, Sports Information Directors and media members from across the country, and is published weekly.