Through games of Jan. 23, 2000:
| No. | Team | W-L | Total | Last |
| 1 | Washington U. (25) | 15-0 | 625 | 1 |
| 2 | Simpson | 16-0 | 598 | 2 |
| 3 | UW-Eau Claire | 16-0 | 570 | 3 |
| 4 | Cortland State | 14-0 | 540 | 4 |
| 5 | Baldwin-Wallace | 16-1 | 520 | 5 |
| 6 | Scranton | 15-1 | 500 | 6 |
| 7 | Rowan | 14-1 | 475 | 7 |
| 8 | St. Thomas | 14-1 | 457 | 9 |
| 9 | UW-Oshkosh | 15-3 | 408 | 10 |
| 10 | Millikin | 15-1 | 381 | 11 |
| 11 | Capital | 14-3 | 367 | 8 |
| 12 | Hardin-Simmons | 16-0 | 351 | 12 |
| 13 | Johns Hopkins | 13-3 | 276 | 16 |
| 14 | Southern Maine | 14-2 | 268 | 18 |
| 15 | NYU | 12-3 | 258 | 13 |
| 16 | New Jersey | 13-3 | 236 | 19 |
| 17 | William Paterson | 13-3 | 193 | 14 |
| 18 | King's (Pa.) | 12-2 | 170 | 15 |
| 19 | William Smith | 14-2 | 154 | 22 |
| 20 | Ohio Wesleyan | 13-2 | 150 | 20 |
| 21 | St. Benedict | 10-4 | 126 | 17 |
| 22 | Pacific Lutheran | 9-4 | 104 | 23 |
| 23 | Trinity (Texas) | 13-3 | 79 | --- |
| 24 | Allentown | 13-2 | 76 | --- |
| 25 | Buffalo State | 12-2 | 64 | 25 |
Dropped out: No. 21 Gwynedd-Mercy, No. 24 Western Connecticut
Others receiving votes: Wellesley 28; DePauw 21; Gwynedd-Mercy 21; Lake Forest 21; Hendrix 16; Williams 13; George Fox 12; Lakeland 12; Bates 11; Concordia-Moorhead 8; McMurry 4; Sewanee 4; Elizabethtown 3; Linfield 3; Hanover 1; Richard Stockton 1.
The D3hoops.com Top 25 is voted on by a panel of 25 coaches, Sports Information Directors and media members from across the country, and is published weekly.