Through games of Feb. 27, 2000:
| No. | Team | W-L | Total | Prev. |
| 1 | Washington U. (25) | 25-0 | 625 | 1 |
| 2 | UW-Eau Claire | 27-0 | 599 | 2 |
| 3 | Baldwin-Wallace | 26-1 | 567 | 3 |
| 4 | St. Thomas | 24-1 | 555 | 4 |
| 5 | Cortland State | 26-1 | 505 | 7 |
| 6 | Hardin-Simmons | 25-1 | 489 | 8 |
| 7 | Capital | 23-4 | 452 | 6 |
| 8 | Millikin | 23-2 | 449 | 5 |
| 9 | Scranton | 23-3 | 360 | 9 |
| 10 | Simpson | 23-4 | 356 | 16 |
| 11 | St. Benedict | 20-5 | 337 | 14 |
| 12 | Allentown | 24-3 | 322 | 19 |
| 13 | UW-Oshkosh | 21-6 | 311 | 12 |
| 14 | Rowan | 22-3 | 303 | 10 |
| 15 | NYU | 20-5 | 293 | 15 |
| 16 | Ohio Wesleyan | 23-3 | 272 | 11 |
| 17 | King's (Pa.) | 22-5 | 231 | 18 |
| 18 | Johns Hopkins | 21-5 | 163 | 23 |
| 19 | New Jersey | 20-5 | 133 | 13 |
| 20 | McMurry | 22-4 | 94 | 22 |
| 21 | George Fox | 21-4 | 76 | --- |
| 22 | Pacific Lutheran | 19-5 | 75 | --- |
| 23 | Wellesley | 21-3 | 74 | 20 |
| 24 | Lakeland | 23-3 | 73 | 17 |
| 25 | Southern Maine | 22-5 | 68 | --- |
Dropped out: No. 21 William Paterson; No. 24 Trinity (Texas); No. 25 William Smith
Others receiving votes: DePauw 57; Hendrix 52; St. Lawrence 37; William Paterson 33; Bates 30; Gwynedd-Mercy 28; Richard Stockton 26; William Smith 18; Wheaton 15; Lake Forest 13; Roanoke 11; Calvin 10; Sewanee 8; Kenyon 3; Hope 1; Salisbury State 1.
The D3hoops.com Top 25 is voted on by a panel of 25 coaches, Sports Information Directors and media members from across the country, and is published weekly.