For the 2021-22 season, Division III realigned basketball into 10 regions. Region 2 is one of two regions made up of former Northeast Region teams. To find the rest of the former Northeast Region teams such as the GNAC, MASCAC, NAC and NESCAC, check out Region 1.
| School | Location | Conference | Enrollment |
| Babson | Babson Park, MA | NEWMAC | 2107 |
| Brandeis | Waltham, MA | UAA | 3709 |
| Castleton | Castleton, VT | LEC | 1718 |
| Clark | Worcester, MA | NEWMAC | 2204 |
| Coast Guard | New London, CT | NEWMAC | 1016 |
| Curry | Milton, MA | CCC | 2101 |
| Eastern Connecticut | Willimantic, CT | LEC | 4287 |
| Emerson | Boston, MA | NEWMAC | 3707 |
| Endicott | Beverly, MA | CCC | 2536 |
| Gordon | Wenham, MA | CCC | 1670 |
| Hartford | West Hartford, CT | CCC | 3681 |
| Keene State | Keene, NH | LEC | 4549 |
| Mass-Boston | Boston, MA | LEC | 7800 |
| Mass-Dartmouth | North Dartmouth, MA | LEC | 6995 |
| MIT | Cambridge, MA | NEWMAC | 4105 |
| Nichols | Dudley, MA | CCC | 1021 |
| Plymouth State | Plymouth, NH | LEC | 4075 |
| Rhode Island College | Providence, RI | LEC | 4443 |
| Roger Williams | Bristol, RI | CCC | 3870 |
| Salve Regina | Newport, RI | NEWMAC | 1931 |
| Southern Maine | Gorham, ME | LEC | 4424 |
| Springfield | Springfield, MA | NEWMAC | 3053 |
| Suffolk | Boston, MA | CCC | 4062 |
| University of New England | Biddeford, ME | CCC | 2049 |
| Wentworth | Boston, MA | CCC | 3418 |
| Western Connecticut | Danbury, CT | LEC | 4599 |
| Western New England | Springfield, MA | CCC | 2497 |
| Wheaton (Mass.) | Norton, MA | NEWMAC | 1545 |
| WPI | Worcester, MA | NEWMAC | 3075 |