For the 2021-22 season, Division III realigned basketball into 10 regions. Region 7 is primarily teams from the former Great Lakes Region.
| School | Location | Conference | Enrollment |
| Adrian | Adrian, MI | MIAA | 1606 |
| Albion | Albion, MI | MIAA | 1552 |
| Alfred State | Alfred, NY | AMCC | 3277 |
| Allegheny | Meadville, PA | PAC | 1748 |
| Alma | Alma, MI | MIAA | 1381 |
| Baldwin Wallace | Berea, OH | OAC | 2933 |
| Bethany | Bethany, WV | PAC | 593 |
| Calvin | Grand Rapids, MI | MIAA | 4075 |
| Capital | Columbus, OH | OAC | 2329 |
| Carnegie Mellon | Pittsburgh, PA | UAA | 5609 |
| Case Western Reserve | Cleveland, OH | UAA | 4196 |
| Chatham | Pittsburgh, PA | PAC | 914 |
| Denison | Granville, OH | NCAC | 2173 |
| DePauw | Greencastle, IN | NCAC | 2237 |
| Franciscan (Ohio) | Steubenville, OH | PAC | 2001 |
| Geneva | Beaver Falls, PA | PAC | 1564 |
| Grove City | Grove City, PA | PAC | 2509 |
| Heidelberg | Tiffin, OH | OAC | 1021 |
| Hilbert | Hamburg, NY | AMCC | 878 |
| Hiram | Hiram, OH | NCAC | 1135 |
| Hope | Holland, MI | MIAA | 3191 |
| John Carroll | University Heights, OH | OAC | 3012 |
| Kalamazoo | Kalamazoo, MI | MIAA | 1406 |
| Kenyon | Gambier, OH | NCAC | 1635 |
| La Roche | Pittsburgh, PA | AMCC | 1179 |
| Marietta | Marietta, OH | OAC | 1396 |
| Mount Aloysius | Cresson, PA | AMCC | 1240 |
| Mount Union | Alliance, OH | OAC | 1978 |
| Muskingum | New Concord, OH | OAC | 1470 |
| Oberlin | Oberlin, OH | NCAC | 2793 |
| Ohio Northern | Ada, OH | OAC | 2545 |
| Ohio Wesleyan | Delaware, OH | NCAC | 1937 |
| Olivet | Olivet, MI | MIAA | 941 |
| Otterbein | Westerville, OH | OAC | 2312 |
| Penn State-Altoona | Altoona, PA | AMCC | 3466 |
| Penn State-Behrend | Erie, PA | AMCC | 3295 |
| Pitt-Bradford | Bradford, PA | AMCC | 1356 |
| Pitt-Greensburg | Greensburg, PA | AMCC | 1658 |
| St. Mary's (Ind.) | Notre Dame, IN | MIAA | 1571 |
| St. Vincent | Latrobe, PA | PAC | 1646 |
| Thiel | Greenville, PA | PAC | 1064 |
| Trine | Angola, IN | MIAA | 2215 |
| Washington and Jefferson | Washington, PA | PAC | 1387 |
| Waynesburg | Waynesburg, PA | PAC | 1570 |
| Wells | Aurora, NY | AMCC | 519 |
| Westminster (Pa.) | New Wilmington, PA | PAC | 1087 |
| Wilmington | Wilmington, OH | OAC | 1192 |
| Wittenberg | Springfield, OH | NCAC | 1238 |
| Wooster | Wooster, OH | NCAC | 1862 |