For the 2021-22 season, Division III realigned basketball into 10 regions. Region 6 is primarily teams from the South Atlantic. To find former South Region teams such as the ASC and SCAC, check out Region 10.
| School | Location | Conference | Enrollment |
| Asbury | Wilmore, KY | IND | 1854 |
| Averett | Danville, VA | ODAC | 890 |
| Belhaven | Jackson, MS | CCS | 1242 |
| Berea | Berea, KY | CCS | 1552 |
| Berry | Mount Berry, GA | SAA | 1898 |
| Birmingham-Southern | Birmingham, AL | SAA | 1272 |
| Brevard | Brevard, NC | USAC | 729 |
| Bridgewater (Va.) | Bridgewater, VA | ODAC | 1629 |
| Centre | Danville, KY | SAA | 1306 |
| Christopher Newport | Newport News, VA | C2C | 4583 |
| Covenant | Lookout Mountain, GA | CCS | 1002 |
| Eastern Mennonite | Harrisonburg, VA | ODAC | 941 |
| Emory | Atlanta, GA | UAA | 6587 |
| Ferrum | Ferrum, VA | ODAC | 1370 |
| Greensboro | Greensboro, NC | USAC | 923 |
| Guilford | Greensboro, NC | ODAC | 1676 |
| Hampden-Sydney | Hampden-Sydney, VA | ODAC | 1098 |
| Hendrix | Conway, AR | SAA | 1402 |
| Huntingdon | Montgomery, AL | CCS | 884 |
| LaGrange | LaGrange, GA | CCS | 765 |
| Lynchburg | Lynchburg, VA | ODAC | 1839 |
| Mary Baldwin | Staunton, VA | USAC | 995 |
| Mary Washington | Fredericksburg, VA | C2C | 3573 |
| Maryville (Tenn.) | Maryville, TN | CCS | 1097 |
| Methodist | Fayetteville, NC | USAC | 1974 |
| Millsaps | Jackson, MS | SAA | 989 |
| N.C. Wesleyan | Rocky Mount, NC | USAC | 1754 |
| Oglethorpe | Atlanta, GA | SAA | 981 |
| Pfeiffer | Misenheimer, N.C. | USAC | 1961 |
| Piedmont | Demorest, GA | CCS | 1131 |
| Randolph-Macon | Ashland, VA | ODAC | 1230 |
| Randolph | Lynchburg, VA | ODAC | 583 |
| Rhodes | Memphis, TN | SAA | 1652 |
| Roanoke | Salem, VA | ODAC | 1773 |
| Sewanee | Sewanee, TN | SAA | 1454 |
| Shenandoah | Winchester, VA | ODAC | 1755 |
| Southern Virginia | Buena Vista, VA | USAC | 682 |
| Virginia Wesleyan | Virginia Beach, VA | ODAC | 1281 |
| Warren Wilson | Asheville, NC | C2C | 706 |
| Washington and Lee | Lexington, VA | ODAC | 1749 |
| William Peace | Raleigh, NC | USAC | 751 |