For the 2021-22 season, Division III realigned basketball into 10 regions. Region 1 is one of two regions made up of former Northeast Region teams. To find the rest of the former Northeast Region teams such as the CCC, LEC, NECC and NEWMAC, check out Region 2.
| School | Location | Conference | Enrollment |
| Albertus Magnus | New Haven, CT | GNAC | 1146 |
| Amherst | Amherst, MA | NESCAC | 1835 |
| Anna Maria | Paxton, MA | GNAC | 866 |
| Bates | Lewiston, ME | NESCAC | 1787 |
| Bowdoin | Brunswick, ME | NESCAC | 1797 |
| Bridgewater State | Bridgewater, MA | MASCAC | 7959 |
| Colby-Sawyer | New London, NH | GNAC | 1315 |
| Colby | Waterville, ME | NESCAC | 1847 |
| Connecticut College | New London, CT | NESCAC | 1871 |
| Dean | Franklin, MA | GNAC | 1382 |
| Eastern Nazarene | Quincy, MA | NAC | 1047 |
| Elms | Chicopee, MA | GNAC | 1003 |
| Emmanuel | Boston, MA | GNAC | 1817 |
| Fitchburg State | Fitchburg, MA | MASCAC | 3395 |
| Framingham State | Framingham, MA | MASCAC | 3872 |
| Hamilton | Clinton, NY | NESCAC | 1890 |
| Husson | Bangor, ME | NAC | 2237 |
| Johnson and Wales | Providence, RI | GNAC | 8246 |
| Lasell | Newton, MA | GNAC | 1054 |
| Lesley | Cambridge, MA | NAC | 1480 |
| Maine Maritime | Castine, ME | NAC | 958 |
| Maine-Farmington | Farmington, ME | NAC | 2095 |
| Maine-Presque Isle | Presque Isle, ME | NAC | 1156 |
| Massachusetts College | North Adams, MA | MASCAC | 1109 |
| Middlebury | Middlebury, VT | NESCAC | 2411 |
| Mitchell | New London, CT | GNAC | 751 |
| Morrisville State | Morrisville, NY | NAC | 2708 |
| New England College | Henniker, NH | GNAC | 792 |
| Norwich | Northfield, VT | GNAC | 1911 |
| NVU-Johnson | Johnson, VT | NAC | 924 |
| NVU-Lyndon | Lyndonville, VT | NAC | 1257 |
| Regis (Mass.) | Weston, MA | GNAC | 932 |
| Rivier | Nashua, NH | GNAC | 905 |
| Salem State | Salem, MA | MASCAC | 6404 |
| St. Joseph (Conn.) | West Hartford, CT | GNAC | 818 |
| St. Joseph's (Maine) | Standish, ME | GNAC | 1026 |
| SUNY Delhi | Delhi, NY | NAC | 3469 |
| SUNY-Canton | Canton, NY | NAC | 2952 |
| SUNY-Cobleskill | Cobleskill, NY | NAC | 2801 |
| SUNYIT | Utica, NY | NAC | 1285 |
| Thomas | Waterville, ME | NAC | 727 |
| Trinity (Conn.) | Hartford, CT | NESCAC | 2181 |
| Tufts | Medford, MA | NESCAC | 5004 |
| Wesleyan | Middletown, CT | NESCAC | 2748 |
| Westfield State | Westfield, MA | MASCAC | 4385 |
| Williams | Williamstown, MA | NESCAC | 1983 |
| Worcester State | Worcester, MA | MASCAC | 3354 |