For the 2021-22 season, Division III realigned basketball into 10 regions. Region 5 is primarily teams from the Middle Atlantic. To find former Middle Atlantic Region teams such as the C2C, check out Region 6. To find former Atlantic Region teams such as the CUNYAC, MAC Freedom and NJAC, check out Region 4.
| School | Location | Conference | Enrollment |
| Albright | Reading, PA | MACC | 1993 |
| Alvernia | Reading, PA | MACC | 1717 |
| Cabrini | Radnor, PA | AEC | 1678 |
| Catholic | Washington, DC | LAND | 3196 |
| Centenary (N.J.) | Hackettstown, NJ | AEC | 1610 |
| Dickinson | Carlisle, PA | CC | 2328 |
| Drew | Madison, NJ | LAND | 1666 |
| Eastern | St. Davids, PA | MACC | 2276 |
| Elizabethtown | Elizabethtown, PA | LAND | 1849 |
| Franklin and Marshall | Lancaster, PA | CC | 2302 |
| Gettysburg | Gettysburg, PA | CC | 2579 |
| Goucher | Towson, MD | LAND | 1230 |
| Gwynedd Mercy | Gwynedd Valley, PA | AEC | 1851 |
| Haverford | Haverford, PA | CC | 1187 |
| Hood | Frederick, MD | MACC | 1325 |
| Immaculata | Immaculata, PA | AEC | 821 |
| Johns Hopkins | Baltimore, MD | CC | 5851 |
| Juniata | Huntingdon, PA | LAND | 1555 |
| Lycoming | Williamsport, PA | LAND | 1307 |
| Marymount | Arlington, VA | AEC | 2065 |
| Marywood | Scranton, PA | AEC | 1668 |
| McDaniel | Westminster, MD | CC | 1584 |
| Messiah | Grantham, PA | MACC | 2826 |
| Moravian | Bethlehem, PA | LAND | 1447 |
| Muhlenberg | Allentown, PA | CC | 2267 |
| Neumann | Aston, PA | AEC | 2060 |
| Salisbury | Salisbury, MD | C2C | 6657 |
| Scranton | Scranton, PA | LAND | 3732 |
| Stevenson | Stevenson, MD | MACC | 2060 |
| Susquehanna | Selinsgrove, PA | LAND | 2132 |
| Swarthmore | Swarthmore, PA | CC | 1532 |
| Ursinus | Collegeville, PA | CC | 1647 |
| Washington College | Chestertown, MD | CC | 1408 |
| Widener | Chester, PA | MACC | 2632 |
| Wilkes | Wilkes-Barre, PA | LAND | 2082 |
| York (Pa.) | York, PA | MACC | 4118 |